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Gene Quantification Book by ,

Gene Quantification Book
  • Author : ,
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Category: Medical
  • Pages : 375
  • ISBN Code: 1461241642

Summary: Geneticists and molecular biologists have been interested in quantifying genes and their products for many years and for various reasons (Bishop, 1974). Early molecular methods were based on molecular hybridization, and were devised shortly after Marmur and Doty (1961) first showed that denaturation of the double helix could be reversed - that the process of molecular reassociation was exquisitely sequence dependent. Gillespie and Spiegelman (1965) developed a way of using the method to titrate the number of copies of a probe within a target sequence in which the target sequence was fixed to a membrane support prior to hybridization with the probe - typically a RNA. Thus, this was a precursor to many of the methods still in use, and indeed under development, today. Early examples of the application of these methods included the measurement of the copy numbers in gene families such as the ribosomal genes and the immunoglo bulin family. Amplification of genes in tumors and in response to drug treatment was discovered by this method. In the same period, methods were invented for estimating gene num bers based on the kinetics of the reassociation process - the so-called Cot analysis. This method, which exploits the dependence of the rate of reassociation on the concentration of the two strands, revealed the presence of repeated sequences in the DNA of higher eukaryotes (Britten and Kohne, 1968). An adaptation to RNA, Rot analysis (Melli and Bishop, 1969), was used to measure the abundance of RNAs in a mixed population.

Real-time PCR Book by M Tevfik Dorak

Real-time PCR Book
  • Author : M Tevfik Dorak
  • Publisher : Garland Science
  • Release Date : 2007-02-08
  • Category: Science
  • Pages : 333
  • ISBN Code: 0203967313

Summary: With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

The Elements of Statistical Learning Book by Trevor Hastie,Robert Tibshirani,Jerome Friedman

The Elements of Statistical Learning Book
  • Author : Trevor Hastie,Robert Tibshirani,Jerome Friedman
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-11-11
  • Category: Mathematics
  • Pages : 536
  • ISBN Code: 0387216065

Summary: During the past decade there has been an explosion in computation and information technology. With it have come vast amounts of data in a variety of fields such as medicine, biology, finance, and marketing. The challenge of understanding these data has led to the development of new tools in the field of statistics, and spawned new areas such as data mining, machine learning, and bioinformatics. Many of these tools have common underpinnings but are often expressed with different terminology. This book describes the important ideas in these areas in a common conceptual framework. While the approach is statistical, the emphasis is on concepts rather than mathematics. Many examples are given, with a liberal use of color graphics. It should be a valuable resource for statisticians and anyone interested in data mining in science or industry. The book’s coverage is broad, from supervised learning (prediction) to unsupervised learning. The many topics include neural networks, support vector machines, classification trees and boosting---the first comprehensive treatment of this topic in any book. This major new edition features many topics not covered in the original, including graphical models, random forests, ensemble methods, least angle regression & path algorithms for the lasso, non-negative matrix factorization, and spectral clustering. There is also a chapter on methods for “wide” data (p bigger than n), including multiple testing and false discovery rates. Trevor Hastie, Robert Tibshirani, and Jerome Friedman are professors of statistics at Stanford University. They are prominent researchers in this area: Hastie and Tibshirani developed generalized additive models and wrote a popular book of that title. Hastie co-developed much of the statistical modeling software and environment in R/S-PLUS and invented principal curves and surfaces. Tibshirani proposed the lasso and is co-author of the very successful An Introduction to the Bootstrap. Friedman is the co-inventor of many data-mining tools including CART, MARS, projection pursuit and gradient boosting.

Genomics Book by Mike Starkey,Ramnath Elaswarapu

Genomics Book
  • Author : Mike Starkey,Ramnath Elaswarapu
  • Publisher : John Wiley & Sons
  • Release Date : 2010-10-19
  • Category: Science
  • Pages : 360
  • ISBN Code: 0470711620

Summary: Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice for immediate use at the bench. Key features: Provides a broad introduction to current practices and techniques for lab-based research in genomics Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by a leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in genomics Includes troubleshooting guide and reviews of alternative techniques An essential laboratory manual for students and researchers at all levels

Rapid Cycle Real-Time PCR Book by S. Meuer,C. Wittwer,K. Nakagawara

Rapid Cycle Real-Time PCR Book
  • Author : S. Meuer,C. Wittwer,K. Nakagawara
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Category: Science
  • Pages : 408
  • ISBN Code: 3642595243

Summary: The first comprehensive treatise on Rapid Cycle Real-Time PCR. With amplification times of 15-30 minutes of on-line detection and analysis, nucleic acid quantification of mutation analysis finally becomes a routine, powerful and rapid method. Focusing primarily on the LightCycler, an instrument that combines Rapid Cycle PCR with fluorescent monitoring, this technology provides convenient analysis by melting temperatures. PCR products can be identified by product Tm, and single base mismatches can easily be genotyped by probe Tm. Methods chapters detail the theory behind quantification of mutation analysis; the design of synthesis of fluorescent hybridization probes of the preparation of template DNA. Application chapters apply nucleid acid quantification to infectious organisms of intracellular messengers and mutation detection to somatic of acquired mutations.

Regulation of Genome Editing in Plant Biotechnology Book by Hans-Georg Dederer,David Hamburger

Regulation of Genome Editing in Plant Biotechnology Book
  • Author : Hans-Georg Dederer,David Hamburger
  • Publisher : Springer
  • Release Date : 2019-08-16
  • Category: Law
  • Pages : 372
  • ISBN Code: 3030171191

Summary: This book provides in-depth insights into the regulatory frameworks of five countries and the EU concerning the regulation of genome edited plants. The country reports form the basis for a comparative analysis of the various national regulations governing genetically modified organisms (GMOs) in general and genome edited plants in particular, as well as the underlying regulatory approaches.The reports, which focus on the regulatory status quo of genome edited plants in Argentina, Australia, Canada, the EU, Japan and the USA, were written by distinguished experts following a uniform structure. On this basis, the legal frameworks are compared in order to foster a rational assessment of which approaches could be drawn upon to adjust, or to completely realign, the current EU regime for GMOs. In addition, a separate chapter identifies potential best practices for the regulation of plants derived from genome editing.

Principles and Technical Aspects of PCR Amplification Book by Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays

Principles and Technical Aspects of PCR Amplification Book
  • Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
  • Publisher : Springer Science & Business Media
  • Release Date : 2008-03-14
  • Category: Science
  • Pages : 330
  • ISBN Code: 9781402062414

Summary: Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Introduction to Proteomics Book by Daniel C. Liebler

Introduction to Proteomics Book
  • Author : Daniel C. Liebler
  • Publisher : Springer Science & Business Media
  • Release Date : 2001-12-04
  • Category: Medical
  • Pages : 198
  • ISBN Code: 1592591302

Summary: Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

The Protein Protocols Handbook Book by John M. Walker

The Protein Protocols Handbook Book
  • Author : John M. Walker
  • Publisher : Springer Science & Business Media
  • Release Date : 2008-02-12
  • Category: Science
  • Pages : 809
  • ISBN Code: 1603272593

Summary: In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Management of Legionella in Water Systems Book by National Academies of Sciences, Engineering, and Medicine,Health and Medicine Division,Division on Earth and Life Studies,Board on Population Health and Public Health Practice,Board on Life Sciences,Water Science and Technology Board,Committee on Management of Legionella in Water Systems

Management of Legionella in Water Systems Book
  • Author : National Academies of Sciences, Engineering, and Medicine,Health and Medicine Division,Division on Earth and Life Studies,Board on Population Health and Public Health Practice,Board on Life Sciences,Water Science and Technology Board,Committee on Management of Legionella in Water Systems
  • Publisher : National Academies Press
  • Release Date : 2020-02-20
  • Category: Medical
  • Pages : 290
  • ISBN Code: 0309493854

Summary: Legionnaires' disease, a pneumonia caused by the Legionella bacterium, is the leading cause of reported waterborne disease outbreaks in the United States. Legionella occur naturally in water from many different environmental sources, but grow rapidly in the warm, stagnant conditions that can be found in engineered water systems such as cooling towers, building plumbing, and hot tubs. Humans are primarily exposed to Legionella through inhalation of contaminated aerosols into the respiratory system. Legionnaires' disease can be fatal, with between 3 and 33 percent of Legionella infections leading to death, and studies show the incidence of Legionnaires' disease in the United States increased five-fold from 2000 to 2017. Management of Legionella in Water Systems reviews the state of science on Legionella contamination of water systems, specifically the ecology and diagnosis. This report explores the process of transmission via water systems, quantification, prevention and control, and policy and training issues that affect the incidence of Legionnaires' disease. It also analyzes existing knowledge gaps and recommends research priorities moving forward.

The GEO Handbook on Biodiversity Observation Networks Book by Michele Walters,Robert J. Scholes

The GEO Handbook on Biodiversity Observation Networks Book
  • Author : Michele Walters,Robert J. Scholes
  • Publisher : Springer
  • Release Date : 2016-11-25
  • Category: Science
  • Pages : 326
  • ISBN Code: 3319272888

Summary: Biodiversity observation systems are almost everywhere inadequate to meet local, national and international (treaty) obligations. As a result of alarmingly rapid declines in biodiversity in the modern era, there is a strong, worldwide desire to upgrade our monitoring systems, but little clarity on what is actually needed and how it can be assembled from the elements which are already present. This book intends to provide practical guidance to broadly-defined biodiversity observation networks at all scales, but predominantly the national scale and higher. This is a practical how-to book with substantial policy relevance. It will mostly be used by technical specialists with a responsibility for biodiversity monitoring to establish and refine their systems. It is written at a technical level, but one that is not discipline-bound: it should be intelligible to anyone in the broad field with a tertiary education.

Molecular Epidemiology Book by Paul A. Schulte,Frederica P. Perera

Molecular Epidemiology Book
  • Author : Paul A. Schulte,Frederica P. Perera
  • Publisher : Academic Press
  • Release Date : 2012-12-02
  • Category: Science
  • Pages : 588
  • ISBN Code: 0323138578

Summary: This book will serve as a primer for both laboratory and field scientists who are shaping the emerging field of molecular epidemiology. Molecular epidemiology utilizes the same paradigm as traditional epidemiology but uses biological markers to identify exposure, disease or susceptibility. Schulte and Perera present the epidemiologic methods pertinent to biological markers. The book is also designed to enumerate the considerations necessary for valid field research and provide a resource on the salient and subtle features of biological indicators.

Principles of Marketing Book by Phil T. Kotler,Gary Armstrong,Lloyd C. Harris,Prof Hongwei He

Principles of Marketing Book
  • Author : Phil T. Kotler,Gary Armstrong,Lloyd C. Harris,Prof Hongwei He
  • Publisher : Pearson Higher Ed
  • Release Date : 2019-11-14
  • Category: Business & Economics
  • Pages : 720
  • ISBN Code: 129226960X

Summary: Principles of Marketing, helps students understand how to create value, build customer relationshipsand master key marketing challenges. The the8th Edition has been thoroughly revised to reflect the major trends impacting contemporary marketing. Packed withexamples illustrating how companies use new digital technologies to maximize customer engagement and shape brand conversations, experiences, and communities.

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents Book by Mark A. Suckow,Karla A. Stevens,Ronald P. Wilson

The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents Book
  • Author : Mark A. Suckow,Karla A. Stevens,Ronald P. Wilson
  • Publisher : Academic Press
  • Release Date : 2011-12-14
  • Category: Science
  • Pages : 1288
  • ISBN Code: 0123809215

Summary: The Laboratory Rabbit, Guinea Pig, Hamster, and Other Rodents is a single volume, comprehensive book sanctioned by the American College of Laboratory Animal Medicine (ACLAM), covering the rabbit, guinea pig, hamster, gerbil and other rodents often used in research. This well-illustrated reference won a 2012 PROSE Award for Best Single Volume Reference in Science from the Association of American Publishers. The book includes basic biology, anatomy, physiology, behavior, infectious and noninfectious diseases, husbandry and breeding, common experimental methods, and use of the species as a research model. With many expert contributors, this will be an extremely valuable publication for biomedical researchers, laboratory animal veterinarians and other professionals engaged in laboratory animal science. 2012 PROSE Award winner for Best Single Volume Reference in Science from the Association of American Publishers One-stop resource for advancements in the humane and responsible care of: rabbit, guinea pig, hamster, gerbil, chinchilla, deer mouse, kangaroo rat, cotton rat, sand rat, and degu Includes up-to-date, common experimental methods Organized by species for easy access during bench research